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. 2019 Dec 18;25(1):8. doi: 10.3390/molecules25010008

Figure 4.

Figure 4

Size distributions before and after the folding of truss-DNA origamis in bulk solution were determined by dynamic light scattering (DLS): the size distributions of (a) staple DNA only, (b) scaffold DNA only, (c) scaffold DNA and staple DNA mixture (scaffold: staple = 1:1), (d) the mixture sample with an elevated temperature up to 70 °C and (e) the annealed DNA mixture sample. Note that the annealed structure is a mixture of well-folded, partially folded and no structure, as shown in Figure 2. The samples were assessed three times for each (shown as the red, green and blue colors). Staple DNAs showed a polydisperse distribution of their size (polydispersity index (PDI) >0.3), however, just mixing the Scaffold and Staple DNAs showed rather packed state (c). The temperature elevating caused a large spread exceeding 1 µm (d). The size distributions (1, 2, and 3 µm) of the microcompartment droplets used in this study suggest that physical confinement restricts the spread of DNA molecules observed in (d).