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Primer pairs sequences of genes used in the qRT-PCRa
| Gene | Gene ID | Forward primer | Reverse primer |
|---|---|---|---|
| NRAS1 | 4893 | TATTCATCTACAAAGTGGTTCTGG | CGGCTGTGGTCCTAAATCTG |
| NFKB1 | 4790 | GAAGGTGGATGATTGCTAAG | TGCTGGAGTTCAGGATAAC |
| IGF1R | 3480 | CGGTAATAGTCTGTCTCATAG | GCCAATAAGTTCGTCCAC |
| CCND1 | 595 | TTCTGTTCCTCGCAGACCTCC | CGATGCCAACCTCCTCAACG |
| ACTB | 60 | AAGATCAAGATCATTGCT | TAACGCAACTAAGTCATA |
Quantitative reverse transcription PCR
