Table 4.

Effects of AFB1 on pH, ammonia-N concentration and VFAs molar proportions after 24-h of incubation

Variables1	AFB1 concentration (µg/ml)			SEM	P-values (contrasts2)
	0	5	10		CTRL vs. AFB1	L	Q
pH	6.32	6.41	6.44	0.015	0.002	0.003	0.169
NH3-N (mmol/ml)	15.7	16.6	17.5	0.22	0.003	0.003	0.876
TVFA (mmol/ml)	66.8	52.8	45.4	2.10	<0.001	0.001	0.242
VFA molar proportion (mmol/100 mmol)
Acetate (A)	66.3	54.3	53.5	0.90	<0.001	<0.001	0.003
Propionate (P)	19.7	27.7	25.8	0.40	<0.001	<0.001	<0.001
Butyrate	9.7	10.8	13.6	0.76	0.388	0.017	0.368
Isovalerate	1.93	4.29	3.30	0.403	0.011	0.069	0.017
Valerate	1.70	2.95	2.94	0.278	0.013	0.029	0.112
P/A	0.30	0.51	0.49	0.007	0.007	<0.001	<0.001

¹ TVFA: Total volatile fatty acids, P/A: Propionate to acetate ratio, VFAs: Volatile fatty acids, AFB1: Aflatoxin B1, SEM: Standard error of the means, ² CTRL: Control, 0 µg/ml of AFB1, L: Linear, and Q: Quadratic