Table 2.
Primers used for the detection of Escherichia coli and DEC pathotypes
| Genes | Pathotypes | Primer sequence (5´ to 3´) | PCR product size (bp) |
Primer Conc. (pmol/μL) |
References |
|---|---|---|---|---|---|
| Stx1 | STEC, EHEC | AGTTAATGTGGTGGCGAAGG CACCAGACAATGTAACCGC |
347 | 5 | |
| Stx2 | STEC, EHEC | TTCGGTATCCTATTCCCGG CGTCATCGTATACACAGGAG |
592 | 4 | |
| Eae | EHEC, tEPEC, aEPEC | CCCGAATTCGGCACAAGCATAAGC CCCGGATCCGTCTCGCCAGTATTCG |
881 | 5 | |
| bfpA | tEPEC | AATGGTGCTTGCGCTTGCTGC GCCGCTTTATCCAACCTGGTA |
324 | 5 | |
| aggR | EAEC | GTATACACAAAAGAAGGAAGC ACAGAATCGTCAGCATCAGC |
254 | 4 | |
| Elt | ETEC | AACGTTCCGGAGGTCTTATG CAACCTTGTGGTGCATGATG |
511 | 3 | |
| Esth | ETEC | TTCACCTTTCCCTCAGGATG ATAGCACCCGGTACAAGCAG |
172 | 4 | |
| Estp | ETEC | ACTGAATCACTTGACTCTTCA TCACAGCAGTAAAATGTGTTGT |
120 | 10 | |
| invE | EIEC | GCAGGAGCAGATCTTGAAG GAAAGGCACGAGTGACTTTC |
208 | 10 | |
| daaC | DAEC | CACTGTGGGCTCCGCGCAAGC CGGTGAGGTTCAGTGTGTAT |
418 | 10 | et al. (1999) |
| uidA | E. coli | AAAACGGCAAGAAAAAGCAG ACGCGTGGTTACAGTCTTGCG |
147 | 10 |
Stx: Shiga-like toxin, eae: Intimine, bfp: Bundle forming pilus, aggR: Transcriptional activator for EAEC virulence genes, elt: Heat labile toxin, esth and estp: Variants of heat stable toxin, invE: Protein for invasion, daaC: Dr family of adhesions (F1845), uidA: Gene encode for B-glucuronidase enzyme, STEC: Shiga-toxigenic Escherichia coli, EHEC: Enterohaemorrhagic Escherichia coli, tEPEC and aEPEC: Typical and atypical enteropathogenic Escherichia coli, EAEC: Enteroaggregative Escherichia coli, ETEC: Entertoxigenic Escherichia coli, EIEC: Enteroinvasive Escherichia coli, DAEC: Diffusely adherent Escherichia coli, and DEC: Diarrheagenic Escherichia coli