. Author manuscript; available in PMC: 2021 Jan 1.

Published in final edited form as: Insect Biochem Mol Biol. 2019 Nov 21;116:103287. doi: 10.1016/j.ibmb.2019.103287

Table 1.

Different critical parameters for multiple reaction monitoring (MRM) detection of the JHs and MF.

Analyte	Ret. time	Parent ion	Primary fragment		Secondary fragment
Analyte	(min)	(m/z)	(m/z)	CID (V)	(m/z)	CID (V)	%
JHB₃	8.60	283	233	10.0	251	7.0	34
JHSB₃	8.94	283	233	9.0	145	18.0	19
JH III^*	9.40	267	235	5.0	147	10.0	29
JH II	9.64	281	249	5.0	231	6.0	14
JH I	9.82	295	263	5.0	161	12.0	25
MF	10.16	251	191	9.0	219	6.0	40

Retention times are in minutes. Collision-induced dissociation (CID) energies are in volts (V). %: represents the relative abundance of secondary fragments as % of the signal intensity of the primary fragment. Qualitative analysis tolerances were ± 0.2 min from retention times and ± 5% of relative abundances of secondary fragments. LC peak width at base was 0.15 min or lower for all compounds.

Detection of JH III-D3 internal standard was done on parent ion m/z 270 with same retention time, fragment ions and collision energies than JH III.