Table 2:

Limits of detection (LOD) for the different JH homologs and MF.

JH Homolog	LOD (pg/ml)	LOD test range (pg/ml)	LOD (fmol on column)	n
JH I	3.5	5-500	0.48	43
JH II	4.0	5-500	0.56	43
JH III	3.6	5-200	0.55	54
JHSB3	7.8	5-500	1.10	51
JHB3	8.7	5-1000	1.23	21
MF	7.0	10-600	1.12	46

Limit of detections (LOD), defined as three times the background analytical response, were determined experimentally for each JH form and MF (pg/ml). Compounds were tested in the LOD test range (pg/ml). The LOD is also expresses as fmol detected on the column. LOD values were based in 21-54 replicates (n)