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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Insect Biochem Mol Biol. 2019 Nov 4;116:103261. doi: 10.1016/j.ibmb.2019.103261

Fig. 2.

Fig. 2.

SDS-PAGE separation and immunoblot analysis of the hemolymph proteins eluted from the serpin-12 (A) or HP14PD (B) antibody columns. As described in Section 2.3, plasma proteins associated with the antibodies were eluted from the column. Aliquots of the peak fractions were separated by 7.5% SDS-PAGE, stained with Coomassie brilliant blue (CBB) (left panel), and analyzed using diluted serpin-12 or HP14PD antiserum (middle and right panels), as indicated at the bottom. Lane 1, eluted proteins from induced plasma not treated with S. aureus peptidoglycan; lane 2, eluted proteins from the induced plasma elicited with peptidoglycan. Positions and sizes (in kDa) of the pre-stained Mr standards are marked on the left, with the 75 kDa marker highlighted red. *, putative serpin-protease complexes; ►, uncoupled IgG heavy chain.