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Stimulation of proPO activation in induced plasma from M. sexta larvae by A. gambiae SP217. Aliquots of IP (1.0 μl) were incubated with SP217 (0.1 μg) in 18 μl of buffer A for 10 min at room temperature before PO activity measurement. As negative controls, IP or SP217 alone were incubated with buffer A under the same conditions. PO activities in the reaction and controls were measured and plotted as mean ± range (n = 2) on the bar graph.
