Fig. 8.

Covalent complexes of A. gambiae SP217 with serpin-12ΔN (A), SRPN11ΔN (B) or SRPN17ΔN (C). To test SP217 inhibition, aliquots of SP217 (50 ng) were separately reacted with serpin-12ΔN (0.15 μg), SRPN11ΔN (1 μg), or SRPN17ΔN (1 μg) in buffer A (12 μl) for 30 min at room temperature. For use as negative controls, the same amounts of SP217 and serpins alone were incubated under the same conditions. The samples were separated by 10% SDS-PAGE under reducing condition and analyzed using the antibody to pentahistidine. *, serpin-protease complex; •, serpin; ►, 30 kDa protease domain of SP217.