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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Hum Genet. 2019 Dec 3;139(2):151–184. doi: 10.1007/s00439-019-02095-5

Fig. 1. Generation of mouse E14.5 lens and whole embryonic body (WB) proteome to identify lens enriched genes.

Fig. 1.

(A) Mouse E14.5 lens and WB (eye tissue removed) were micro-dissected and 55 µg protein of each sample of lens and WB (n=5 samples for each of lens and WB) was processed using high-throughput mass spectrometry. Differential protein expression was analyzed by comparing with whole embryonic body (WB) reference proteome. (B) Flow chart showing the pipeline that was followed for differential protein expression analysis. Normalized spectral counts were subjected to differential protein expression using edgeR pipeline. Proteins with false discovery rate (FDR) < 0.01 and fold change (FC) ≥ 2 were considered lens enriched.