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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Hum Genet. 2019 Dec 3;139(2):151–184. doi: 10.1007/s00439-019-02095-5

Fig. 2. Quality assessment of MS/MS data of lens and WB.

Fig. 2.

(A) Multidimensional scaling analysis showed that individual biological replicates of the lens and WB samples clustered together while the overall lens and WB samples clustered separately from each other. The axes show the leading dimensions 1 and 2. (B) Spectral counts in WB and lens samples were subjected to TMM (trimmed mean of M-values) normalization using edgeR to correct for the dramatic compositional differences. The boxplots for the normalized SpC datasets showed comparable median SpCs between the lens and the WB samples. The y-axis represents the TMM normalized SpC. (C) A scatter matrix was generated for five lens samples and correlation was examined for the sample to sample consistency. The lens samples showed a high correlation. (D) A scatter matrix was generated for five WB samples and correlation was examined for the sample to sample consistency. The WB samples showed a high correlation. (E) A scatter plot with regression analysis shows no correlation (r = 0.4919) between the average lens and average WB samples.