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Pause positions stalled by AMP-PNP. (A) Experimental procedure. After observing binding dwell at 100 nM [ATP] (Left), 500 nM AMP-PNP and 100 nM ATP was introduced into the reaction mixture (Right). Blue data points represent the positions when rotation was blocked with AMP-PNP. (B) The angular distance (Δθ) of AMP-PNP inhibition from the nearest binding dwell (pink) (n = 27). Values are mean ± SD.
