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. 2019 Dec 23;117(3):1533–1542. doi: 10.1073/pnas.1915311117

Fig. 3.

Fig. 3.

Evolution of IRE1 clusters under sustained ER stress. IRE1-mNG cells were treated with 5 μg/mL Tm for the indicated times, then fixed, imaged, and analyzed as described in the text to plot (A) fraction of cells with 1 or more IRE1 clusters, (B) fraction of total IRE1 inside clusters, (C) number of clusters per cell with 1 or more clusters, and (D) cluster intensity. All plots show mean values and 68% CI. Number of cells analyzed for each condition, in ascending order: 178, 164, 149, 207, 177, 146, 163, 134. (E) Clustering time course extracted from live imaging of Tm-treated IRE1-mNG cells. Only cells that start off with no clusters at t = 0, form multiple clusters upon addition of Tm, and dissolve all clusters by the end of the experiment are used to construct the time course. Number of clusters per cell is plotted in blue and mean cluster intensity is plotted in orange. Data are binned and shown as mean with 95% CI (n = 1,601 cells). A.U., arbitrary units.