Fig. 2.

NLRP3 activates the EBV replication switch via TXNIP. (A–D) HH514-16 cells were transfected with (A) TXNIP-directed (or control) siRNA, (B) TXNIP (or control) plasmid, and (C and D) combinations of plasmids and siRNAs, exposed to NaB 18 h later, and harvested after another (A and B) 12 h or (C and D) 24 h for immunoblotting with indicated antibodies. Numbers represent ratios between indicated proteins. (E) PBMC from healthy subjects (controls [C]) and NOMID patients (N) were infected with EBV in the presence of FK506 and analyzed 24 h later for transcripts from EBV lytic cycle genes BZLF1, BMRF1, and BFRF3 by qRT-PCR. Transcript levels in each sample were compared to those in control sample 1. (F) Three-week-old EBV-transformed LCL derived from healthy subjects (C) and NOMID patients (N) were harvested for analysis of EBV lytic transcripts BZLF1, BMRF1, and BLLF1 by qRT-PCR. Data represent averages of three independent experiments; error bars, SEM; *P ≤ 0.05.