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. 2020 Jan 8;117(3):1404–1413. doi: 10.1073/pnas.1914069117

Fig. 7.

Fig. 7.

Effect of bioderivatization on product titer, yield, and cellular growth of engineered Synechocystis sp. PCC 6803. (A) Biomass accumulation of Δaas-PnrsB-Sfp-CAR-Pcoa-‘CpFatB1-4 and Δaas-PnrsB-Sfp-CAR-Pcoa-‘CpFatB1-4-SAAT cultivated in the absence of overlay for 20 d. All strains were induced to express recombinant proteins on day 2 with 625 nM cobalt and 15 µM nickel. After 20 d of cultivation in the absence of overlay, each culture was used to inoculate fresh cultures with overlay and induced to express recombinant proteins as above. (B and C) Photographs of the 1-octanol strain (−AAT) and octyl acetate strain (+AAT) on day 10 when cultivated in the absence (B) or presence (C) of 30% (vol/vol) hexadecane solvent overlay. (D–F) Biomass accumulation (D), product titer (E), and product yield (F) in solvent overlay cultures sampled on day 16. (G) Localization of 1-octanol from a 1-octanol–producing strain with solvent overlay sampled on day 10. Student’s t test analyses on all data shown in D–F were statistically significant (P ≤ 0.01).