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Intracellular cytokine levels in IRF5 or IRF4 CKO microglia by flow cytometry performed on stroke and sham brains. Quantification data are presented as mean MFI. (A–L) Data of IRF4 and IRF5 CKO microglia, respectively. A, D, G, and J are representative intracellular staining plots for TNFα/IL-1β and IL-4/IL-10. MFI of these cytokines were quantified in B–F for IRF5 CKO and in H, I, K, and L for IRF4 CKO microglia. n = 4 to 5 per sham and 6 to 7 per stroke group; *P < 0.05.
