Fig. 3.

Tagmentation-based approach for eccDNA analysis as a verification of the restriction enzyme method. Plasma DNA samples from five pregnancy cases were analyzed by tagmentation. (A) Plasma eccDNA counts detected in pregnancy cases by MspI digestion and tagmentation. P < 0.05, Wilcoxon rank-sum test. EPM, eccDNA per million mappable reads. (B) Size distributions of eccDNA detected by MspI digestion (blue) and tagmentation (red). (C and D) Plots of the size distributions of maternal- and fetal-derived eccDNA, respectively. (E) Cumulative frequency plots of maternal- (blue) and fetal-derived (red) plasma eccDNA.