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. 2019 Dec 17;117(3):1395–1403. doi: 10.1073/pnas.1914380117

Fig. 4.

Fig. 4.

EmtA is involved in the anaerobic estrogen catabolism in Denitratisoma sp. strain DHT3. (A) Confirmation of intragenic insertion of the group II intron (∼2 kb) into the emtA of the emtA-disrupted mutant (emtA) using PCR with the primers flanking this gene. (B) Testosterone and estradiol utilizations by the wild type or emtA-disrupted mutant of strain DHT3 (emtA). (C) Phylogenetic relationship of EmtA and other cobamide-dependent methyltransferases. The phylogenetic tree was constructed using the neighbor-joining method with Jukes–Cantor parameter and a bootstrap value of 1,000. The asterisk (*) represents the terminal methyl acceptors for MetH and EmtA.