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. 2020 Jan 2;117(3):1321–1329. doi: 10.1073/pnas.1915202117

Fig. 6.

Fig. 6.

Protein deubiquitinase UCHL3 as a target of AuMesoIX. (A) Thermal stabilization of UCHL3 in AuMesoIX-treated A2780 cells as determined by CETSA. (B) List of the m/z of adducts formed between AuMesoIX and peptides derived from DUBs containing the catalytic amino acid residues (cysteine, aspartic acid, or histidine) as determined by ESI-QTOF-MS. (C) DUB inhibitory activity of AuMesoIX (0.5 μM) toward purified enzymes (UCHL1, 3 or 5; 0.4 μg) and A2780 cellular lysate (5 μg). (D) Immunoblot analysis of ubiquitinated protein accumulation in cells treated with AuMesoIX for 18 h. MG132 treatment (5 μM) was used as a positive control.