Fig. 4.

klu expression in EBs and its loss-of-function phenotype. (A–C) Coexpression of klu and the EB reporter Su(H)GBE-LacZ. (D–F′). Knockdown of klu in ISCs/EBs results in an increase of EEs, marked by pros staining: red, pros; green, esg; blue, DAPI. (G) Quantification of EE number. Data are represented as mean ± SEM. Two-tailed t test, ****P < 0.0001. (H) qRT-PCR measurement of AstA and Tk expression from midguts expressing klu RNAi in adult ISCs/EBs for 7 d. rp49 was used for normalization. Data are represented as mean ± SEM. Two-tailed t test, **P = 0.0011 for control vs. RNAi-1; **P = 0.0047 for control vs. RNAi-2.