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. 2020 Jan 27;7:6. doi: 10.1186/s40580-019-0214-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — 774A.1 macrophages were incubated for 2 h at 37 °C with medium only or with medium containing 2.0 μM paramagnetic and Rhodamine B-labeled discoidal HDL (dHDL) or spherical HDL mimicking NPs synthesized using a 1:1 mixture of oxidized synthetic apoA-I peptides H4 and H6. a Fluorescence intensities of cell lysates were measured and normalized to total cell protein content. b Loosely packed cell pellets were generated and T1 values were measured using T1-weighted MR imaging of cell pellets incubated with medium alone, dHDL mimicking NPs or sHDL mimicking NPs. c Normalized enhancement ratio (NER) values for cell pellets are calculated from corresponding T1-weighted images and are relative to cells incubated with medium only. d Contrast-to-noise ratio (CNR) values for cell pellets are calculated from the corresponding T1-weighted images and are relative to cells incubated with medium only. e Differential interference contrast (DIC) image of a single representative J774A.1 macrophage incubated with sHDL (similar images were obtained for dHDL). 4′,6-diamino-2-phenylindole (DAPI) dye (blue), Dylight 488-labeled peptide H4 (green), Rhodamine B-labeled lipid (red) and merged image. f Representative axial T1-weighted images of ApoE^−/− mice, collected before (Pre) and 4 h, 24 h, 48 h and 72 h after administered with the equivalent of 0.05 mmol Gd kg⁻¹ of paramagnetic and Rhodamine B-labeled dHDL or sHDL mimicking NPs. The insets in each scan show the original images, which were cropped and enlarged to highlight the aorta. g NER values (mean ± SD) of mouse aorta wall following contrast administration were calculated for each of the time points (n = 5 slices) for the representative mouse using the corresponding T1-weighted images and are relative to muscle. h CNR values (mean ± SD) of mouse aorta wall following contrast administration were calculated for each of the time points (n = 5 slices) using the corresponding T1-weighted images and are relative to muscle.

Reproduce with permission [62]. Copyright 2015, Public Library of Science