Table 2.
Peptide | Theoretical (Da) | MALDI-TOF (Da)a | AUC (Da)b | |
---|---|---|---|---|
Apo-peptides | Peptide + K2hPg | |||
VEK50 | 6,159 | 6,166 | 6,300 ± 800 | 27,300 ± 850 |
VEK50ΔRH1 | 6,008 | 6008 | 5,600 ± 500 | 16,150 ± 1,000 |
VEK50ΔRH2 | 6,008 | 6010 | 5,700 ± 600 | 16,400 ± 1,000 |
K2hpg | 10,151 | 10,155 | 10,260 ± 800 | above |
Molecular masses of the protein linear sequence from MALDI-TOF.
Weight-average molecular mass of proteins in solution from AUC experiments at 25° C.