FIGURE 2.

Comparison of the performance of ELISA between the newly developed kit and the conventional method. Granulocyte–macrophage colony-stimulating factor autoantibody (GMAb) concentrations measured a) by the modified conventional ELISA kit or b) by the newly developed ELISA kit in the sera from 90 healthy subjects that were previously incubated with or without excess recombinant granulocyte–macrophage colony-stimulating factor (GM-CSF) (50 mg·mL⁻¹). c) The correlation coefficient of the concentrations between the newly developed and the conventional methods was 0.80 in the healthy subjects and 0.95 in the patients with autoimmune pulmonary alveolar proteinosis. d) Receiver operating characteristic curve analysis (area under the curve 1.0).