. 2019 Nov 25;82(1):1–8. doi: 10.1292/jvms.19-0071

Table 1. List of primers and thermal cycling profiles used in this study.

Target	Primer name	Oligonucleotide sequence	Reference strains for standard curves	PCR profile
All Bacteria	341f	CCTACGGGAGGCAGCAG	Escherichia coli JCM 1649T	95°C (5 sec)–60°C (20 sec)–72°C (20 sec)/35 cycles
All Bacteria	534r	ATTACCGCGGCTGCTGG	Escherichia coli JCM 1649T	95°C (5 sec)–60°C (20 sec)–72°C (20 sec)/35 cycles

Actinobacteria	Act920F3	TACGGCCGCAAGGCTA	Bifidobacterium longum subsp. Longum	95°C (20 sec)–54°C (20 sec) –72°C (50 sec)/35 cycles
Actinobacteria	Act1200R	TCRTCCCCACCTTCCTCCG	JCM 1217T	95°C (20 sec)–54°C (20 sec) –72°C (50 sec)/35 cycles

Bacteroidetes	Bact934F	GGARCATGTGGTTTAATTCGATGAT	Bacteroides fragilis DSM 2151T	95°C (5 sec)–55°C (30 sec) /35cycles
Bacteroidetes	Bact1060R	AGCTGACGACAACCATGCAG	Bacteroides fragilis DSM 2151T	95°C (5 sec)–55°C (30 sec) /35cycles

Fusobacteia	Fusobacteria F	GATCCAGCAATTCTGTGTGC	Fusobacterium nucleatum subsp. Nucleatum JCM 8532T	95°C (5 sec)–55°C (20 sec)–72°C (50 sec)/35 cycles
Fusobacteia	Fusobacteria R	CGAATTTCACCTCTACACTTGT	Fusobacterium nucleatum subsp. Nucleatum JCM 8532T	95°C (5 sec)–55°C (20 sec)–72°C (50 sec)/35 cycles

Clostridium cluster XIV	CXIV-F1	GAWGAAGTATYTCGGTATGT	Clostridium clostridioforme JCM 1291T	95°C (5 sec)–52°C (30 sec)–72°C (30 sec)/35 cycles
Clostridium cluster XIV	CXIV-R2	CTACGCWCCCTTTACAC	Clostridium clostridioforme JCM 1291T	95°C (5 sec)–52°C (30 sec)–72°C (30 sec)/35 cycles

Bacteroides spp.	HuBac594Bhqf m	GTTGTGAAAGTTTGCGGCTCAACC	Bacteroides fragilis DSM 2151T	95°C (5 sec)–60°C (30 sec)/35 cycles
	HuBac692r	CTACACCACGAATTCCGCCT	Bacteroides fragilis DSM 2151T	95°C (5 sec)–60°C (30 sec)/35 cycles

Bifidobacterium spp.	BifiLM26F	GATTCTGGCTCAGGATGAACGC	Bifidobacterium longum subsp. JCM1217T	95°C (5 sec)–60°C (20 sec)–72°C (20 sec)/35 cycles
Bifidobacterium spp.	Bif228R	CTGATAGGACGCGACCCCAT	Bifidobacterium longum subsp. JCM1217T	95°C (5 sec)–60°C (20 sec)–72°C (20 sec)/35 cycles

Fusobacterium spp.	*	*	*	95°C (5 sec)–64°C (30 sec) /35cycles

Lactobacillus spp.	LactoR’F	CACAATGGACGMAAGTCTGATG	Lactobacillus casei JCM 1134T	95°C (20 sec)–56°C (20 sec) –72°C (50 sec)/35 cycles
Lactobacillus spp.	LBFR	CGCCACTGGTGTTCTTCCAT	Lactobacillus casei JCM 1134T	95°C (20 sec)–56°C (20 sec) –72°C (50 sec)/35 cycles

Sutterella spp.	**	**	**	95°C (5 sec)–57°C (20 sec)–72°C (20 sec)/35 cycles

Clostridium perfringens	Cperf 165F	CGCATAACGTTGAAAGATGG	Clostridium perfringens JCM 1290T	95°C (5 sec)–60°C (30 sec) /35cycles
Clostridium perfringens	Cperf269R	CCTTGGTAGGCCGTTACCC	Clostridium perfringens JCM 1290T	95°C (5 sec)–60°C (30 sec) /35cycles

Faecalibacterium prausntzii	FPR-2F	GGAGGAAGAAGGTCTTCGG	Faecalibacterium prausnitzii ATCC 27768T	95°C (5 sec)–57°C (20 sec)–72°C (50 sec)/35 cycles
Faecalibacterium prausntzii	Fprau645R	AATTCCGCCTACCTCTGCACT	Faecalibacterium prausnitzii ATCC 27768T	95°C (5 sec)–57°C (20 sec)–72°C (50 sec)/35 cycles

*Fusobacterium Detection Kit (TechnoSuruga Laboratory, Shizuoka, Japan). **Sutterella Detection Kit (TechnoSuruga Laboratory).