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. 2020 Jan 22;147(2):dev180018. doi: 10.1242/dev.180018

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© 2020. Published by The Company of Biologists Ltd

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Fig. 3. — Ars2 is required for neuronal maturation but not for neuronal specification. (A) P12 control (hGFAP-Cre; Ars2^fl/+) and hGFAP-Cre; Ars2^fl/fl brains immunostained for Ars2 (green), BrdU (red) and DAPI (blue). DG, dentate gyrus. (B) P12 brain immunostained for Ars2 (green), NeuN (red) and DAPI (blue). Ars2-knockout hippocampal pyramidal cells (arrowheads) fail to differentiate into mature neurons. (C) Summary of neural development and stage-specific molecular markers. (D-F) P12 hippocampal CA1 region demonstrates that Ars2-knockout cells inappropriately maintain neuroblast/newborn neuronal markers DCX (D) and TBR1 (E), and express BEND6 (F). (G) Immunostaining for cleaved caspase-3 (red) reveals sporadic cell death preferentially in Ars2 null regions. Arrowheads indicate mosaic Ars2-knockout regions in the hippocampal stratum pyramidal layer. Scale bars: 100 μm.