Fig. 7.

Ars2 exhibits broad genomic co-localization with NSC factor SOX2. (A) De novo motif analysis of Ars2 ChIP peaks identified a SOX2/3/6 consensus as the top enriched motif. (B) The SOX2 motif is highly represented in all categories of Ars2-bound regions classified by ChIP-seq enrichment, but especially enriched amongst highest-ranked Ars2 peaks. (C) SOX2 motifs are located close to Ars2 peak summits. (D) SOX2 ChIP-seq data was generated from neurospheres, and overlap analysis of peak calls reveals broad co-localization between SOX2 and Ars2. (E) GO analysis of SOX2 bound regions. (F) Overlap analysis of SOX2 and Ars2 peaks shows enrichment of co-binding amongst the stronger SOX2 peaks. (G) Distribution of Ars2 and SOX2 at representative NSC genes, Ascl1 and Egfr, in proliferating and differentiated neurospheres. The JASPAR SOX2 motif and corresponding sequence are shown. (H) Ars2 and SOX2 co-targeting regions were validated by ChIP-qPCR (red bars in panel G indicate amplicon sites) with our independent Ars2 antibody, i.e. different from the one used for Ars2 ChIP-seq. Materials were treated with RNase before PFA fixation and ChIP. Data are mean±s.e.m.