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. 2020 Jan 23;133(2):jcs232470. doi: 10.1242/jcs.232470

Fig. 3.

Fig. 3.

Mass spectrometric analysis of differentially regulated peptides in senescent versus pre-senescent MSCs. Comprehensive proteomic analysis was performed to find significantly (q value <0.05) changed peptides between the senescent and pre-senescent MSCs (n=4). The overall list of pathway specific genes was examined using gene ontology (GO) cellular component analysis. Differentially altered proteins are highlighted in gray in volcano plots, and critical genes are color-coded based on function. Log2 fold changes (FC) are also tabulated for key dysregulated genes. (A) Proteomic analysis was used to identify Log2 fold changes in the expression of cytoskeleton and nucleoskeleton related proteins for senescent versus pre-senescent MSCs, including actin-related proteins (magenta), microtubules and intermediate filaments (IFs) (red), and nucleoskeleton (blue). (B) Analysis of ECM related proteins was used to identify Log2 fold changes in expression of collagens, and other ECM proteins and crosslinkers for senescent MSCs compared to pre-senescent MSCs. Critical genes for ECM synthesis and turnover are highlighted in the volcano plot, including collagen isoforms (red), crosslinking proteins (blue), and MMP inhibitors (green). It is notable that POSTN, COL1A1, COL14A1 and TIMP3 are among the most highly upregulated proteins.