Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 8;147(1):dev181099. doi: 10.1242/dev.181099

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 5. — Gut morphogenesis is disrupted in cytokinesis mutants. Apical surface staining after E8-E16 division and polarization. (A) ERM-1 apical staining (dashed rectangle) in wild-type (WT) bean-stage embryos. Maximum z-projected images of ERM-1 and nuclei color-coded according to z-depth (scale shown in F) show tissue organization. (B-D) In air-2(or207) embryos, apical surfaces are mispositioned (B-D), branched (B), contain gaps (C) or have broader staining (D). (E) Quantification of the defective apical z-plane distribution in different mutants (more colors indicate greater distortion in the z-plane). (F) ERM-1 staining and distribution of nuclei in a control embryo (GFP degrader only expressed) shows normal lumen width (1.15±0.11 µm, n=10) and nuclear distribution. (G) Endogenous AIR-2::GFP degradation in the intestine results in significantly broadened ERM-1 staining (2.53±0.33 µm, n=8) and disorganized nuclei. Scale bars: 10 μm.