Fig. 3.
Migratory stream integrity partially recovers after removal of JNK inhibitor. (A-C) Migratory streams remain intact (dashed lines, C) when slices are grown in control conditions for 24 h. (D-F) MZ and SVZ streams are disrupted when slices are grown in 20 μM SP600125. (G-I) Partial recovery of the SVZ stream is observed when slices are grown in 20 μM SP600125 for 12 h, rinsed and then grown in control media for an additional 12 h (‘washout’). Representative interneurons are highlighted in red. (J) Quantification of leading process angles. Differences are found between control (129 cells) and 20 μM SP600125 (178 cells; P=0.031), but not between the control and washout (106 cells; P=0.065) conditions (χ2 test). (K,L) Stream dispersion partially recovers in the washout condition (two-way ANOVA; entry zone F(18,120)=2.579, P=0.0012; leading edge F(18,120)=2.785; P=0.0005). All analyses were performed on n=5 brains/condition from 5 experimental days. Data are mean±s.e.m.. ***P<0.001, **P<0.01, *P<0.05, Fisher's LSD post-hoc test. Scale bars: 250 μm in A,D,G; 75 μm in B,C,E,F,H,I.