Figure 6.

Stability of photosynthetic complexes in WT, var2–5, evr3–1, and var2–5 evr3–1 under high light. A, leaf discs from the seventh or eighth true leaves of 4-week-old plants infiltrated with translation inhibitors lincomycin and cycloheximide were treated with high light (∼1000 μmol m⁻² s⁻¹). Amounts of photosynthetic proteins after 0-, 2- and 4-hour high light treatment were analyzed with immunoblots. Antibodies against D1, D2, CP47, and CP43 for PSII, PsaD, and PsaF for PSI, LhcA1, LhcA2, and LhcB2 for antenna complexes, Cytf for cytochrome b₆f complex were used. B, quantifications of immunoblots shown in A. In each blot, protein band signal intensity at 0 h high light treatment was defined as 100% for each genotype. Loadings were normalized to the levels of Cytf. Data were presented as mean ± S.D. of three blots obtained from independent biological replicates.