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. 2019 Dec 10;295(4):1077–1090. doi: 10.1074/jbc.RA119.011424

Figure 4.

Figure 4.

The 49-aa motif in the C terminus increases Nav1.9 current amplitude in HEK293 cells. A–F, representative current traces recorded from HEK293 cells expressing either Nav1.9 (A, green), Nav1.9-CtNav1.7 (B, orange), Nav1.9-Ct49aaNav1.7 (C, navy blue), Nav1.7 (E, red), or Nav1.7-Ct49aaNav1.9 (F, magenta). Current traces recorded from HEK293 cells expressing Nav1.9-Ct49aaNav1.7 and demonstrating ultra-slow inactivation kinetics and large persistent current (in ∼20% HEK293 cells) similar to the parent Nav1.9 current are shown separately (D, light blue). G, comparison of current density among Nav1.9 (n = 15), Nav1.7 (n = 11), and chimera Nav1.7-Ct49aaNav1.9 (n = 10). H, comparison of current density among Nav1.9 (n = 15), Nav1.9-CtNav1.7 (n = 8), and Nav1.9-Ct49aaNav1.7 (n = 7) channels. Error bars, S.E. Statistical significance is indicated by asterisks: *, p < 0.05; **, p < 0.01; ***, p < 0.001, by one-way ANOVA followed by Tukey's multiple-comparison test. ns, not significant.