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. 2019 Dec 16;295(4):1056–1065. doi: 10.1074/jbc.RA119.008854

Figure 1.

Figure 1.

The sensitivities of SMARCAD1-knockout cells to different types of DNA-damaging agents. A, expression of SMARCAD1 and mismatch repair (MMR) proteins in MLH1- and SMARCAD1-knockout cells. The whole-cell extracts were subjected to immunoblotting to detect SMARCAD1 and (MMR) proteins using specific antibodies. β-Actin was a loading control. The molecular weights (× 10−3) are indicated on the left of the panels. B, survival fraction of three types of cell lines after MNU treatment. The cells were treated with MNU for 1 h and then incubated in a drug-free medium for 10 days. The number of colonies was counted, and the survival fractions were determined. Values are the means of at least three independent experiments, and error bars represent S.E. *, significant differences (p < 0.05). C, survival fraction of SMARCAD1-knockout cells treated with other DNA damaging reagents. The cells were treated with etoposide for 12 h or H2O2 for 1 h. For UV irradiation, the cells were exposed to different doses of UV-C. The mean values obtained from at least three independent experiments and the S.E. values are shown. *, significant differences (p < 0.05).