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. 2019 Dec 16;295(4):1056–1065. doi: 10.1074/jbc.RA119.008854

Figure 2.

Figure 2.

The effects of SMARCAD1 knockout on the activation of DNA damage signaling. A, activation of DNA damage signaling after exposure to MNU. Three types of cell lines were treated with 0.2 mm MNU for 1 h and then collected at the indicated times. The whole-cell extracts were prepared and subjected to SDS-PAGE, followed by immunoblotting using the specific antibodies indicated. β-Actin was the loading control. The molecular weights (× 10−3) are indicated on the left of the panels. B, relative intensities of signals for the phosphorylation of ATR, CHK1, ATM, and CHK2. The intensities of the bands for pATR, pCHK1, pATM, and pCHK2 at 48 h after MNU treatment were quantified, and the relative intensities compared with that of HeLa MR are shown. The mean values obtained from three independent experiments and the S.E. values (error bars) are shown. *, significant differences (p < 0.01).