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Increased mutation frequency after MNU treatment. HeLa MR and SMARCAD1-knockout cells were treated with 0, 15, or 30 μm MNU. After the treatment, the numbers of viable cells and ouabain-resistant cells were determined, and the mutation frequencies were calculated. The mean values obtained from three experiments and the S.E. values (error bars) are presented. *, significant differences (p < 0.05).
