Table 4.

Reporting antibody validation results for Western blotting

Antibody details

Vendor/supplier

Antibody name

Catalogue and clone numbers

Species of origin

Immunogen

Lot or batch number

Research Resource Identification (RRID) (if available)

Validation strategy

Genetic, orthogonal, and/or other verification

Positive and negative controls

Antibody titration and optimization

Replication

Samples

Source (e.g. organism, model, cell type, or line)

Endogenous, purified, tagged, or overexpressed target protein

Dose, time, activator/inhibitor, or other treatment conditions

Sample preparation (e.g. lysis conditions, no. of cells)

Validation methods

Amount of sample loaded

Western blotting conditions (including blocking reagent, primary and secondary antibody concentrations, and incubation times)

Detection/visualization methods