Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Suggested benchmarks for user validation of antibody performance in Western blotting
| Specificity |
| ✓Verify by knockdown/knockout of target expression |
| ✓ Appropriate positive and negative controls |
| Selectivity |
| ✓ Detect target at endogenous levels in a complex sample |
| ✓ Optimize antibody dilutions, assay conditions |
| Reproducibility |
| ✓ Repeat/reproduce the experimental result |
| ✓ Confirm observed effect with a complementary method |
