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Negative-stain EM of full-length neurofibromin. A, representative transmission electron micrograph of full-length neurofibromin protein. Scale bar (100 nm) is shown for comparison. B, representative 2D class averages obtained from 41,951 particles. Each box shown is 50 × 50 nm. C, 20-Å density map of full-length neurofibromin generated from 294 class averages shown in multiple orientations. Proposed protomers are indicated with green and blue coloring.
