Table 1.
Neurofibromin proteins used in this study
Cited are the amino acid regions present in purified protein domains, along with the predicted isoelectric point (pI) and the predicted molecular mass of each final protein. Also shown is the construct from which the proteins were purified and the host organism used for protein expression. In the case of His6-MBP fusion proteins, the final purified proteins were cleaved from the MBP solubility tag, and the full-length His6-tagged neurofibromin remained tagged after purification. Constructs C* and C*D have altered start sites within the C domain compared with other C domain–containing proteins.
| Domain | Amino acids | pI | Mass (kDa) | Exp. host | Construct |
|---|---|---|---|---|---|
| Full length | 2–2818 | 6.9 | 317 | Tni-FNL | His6-tev-NF1 |
| ABC | 2–1540 | 6.4 | 173 | Tni-FNL | His6-MBP-tev-NF1(2–1540) |
| ABCD | 2–1820 | 6.5 | 206 | Tni-FNL | His6-MBP-tev-NF1(2–1820) |
| CDEF | 861–2818 | 6.9 | 220 | Tni-FNL | His6-MBP-tev-NF1(861–2818) |
| DEF | 1541–2818 | 8.0 | 144 | Tni-FNL | His6-MBP-tev-NF1(1541–2818) |
| EF | 1821–2818 | 8.1 | 111 | Tni-FNL | His6-MBP-tev-NF1(1821–2818) |
| C*D | 1085–1816 | 6.4 | 83 | Tni-FNL | His6-MBP-tev-NF1(1085–1816) |
| D | 1541–1820 | 7.3 | 32 | Tni-FNL | His6-MBP-tev-NF1(1541–1820) |
| C* | 1085–1530 | 6.0 | 50 | Tni-FNL | His6-MBP-tev-NF1(1085–1530) |
| GRD | 1172–1530 | 6.0 | 41 | E. coli | His6-MBP-tev-NF1(1172–1530) |
| GRD | 1198–1530 | 6.4 | 38 | E. coli | His6-MBP-tev-NF1(1198–1530) |
| GRD | 1203–1530 | 6.6 | 38 | E. coli | His6-MBP-tev-NF1(1203–1530) |