Fig. 1.

Viability of lamina propria cells isolated using starting and optimised methods. C57BL/6 mice were infected with 200 L3 H. polygyrus. 14 days later the small intestine was removed, and LP leukocytes isolated and analysed by flow cytometry. (A) Representative scatter characteristics and (B) representative viability of starting (left) and optimised (right) protocols. (C) % Live cells and (D) Absolute cell numbers of small intestine LP samples from naïve and infected mice, from starting and optimised methods. Graphed data are shown with means ±1 SD and are representative of 3 separate experiments with n = 4–5 in each experiment. Statistical significances calculated by Mann Whitney U test (Significance p < .05, *p < .05).