Table 1.
List of Proteins Displaying at Least 2-Fold Changes in F-Actin Interactome Abundance between Interphase and Metaphase
| Protein name | Normalized total Spectral Count |
Mean PAI |
|||
|---|---|---|---|---|---|
| MS1 |
MS2 |
MS1-MS2 | |||
| G2 | Metaphase | G2 | Metaphase | ||
| Enriched in interphase | G2 PAI | ||||
| TPM4 | 87 | 0 | 253 | 103 | 515 |
| TPM2 | 79 | 0 | 143 | 0 | 335 |
| MYH9 | 627 | 99 | 549 | 15 | 259 |
| TBB3 | 106 | 0 | 72 | 0 | 178 |
| CALD1 | 103 | 1 | 96 | 1 | 163 |
| TBA4A | 92 | 27 | 52 | 26 | 145 |
| TBB6 | 48 | 0 | 35 | 0 | 83 |
| PPR18 | 32 | 14 | 29 | 11 | 45 |
| MYH10 | 94 | 0 | 95 | 0 | 41 |
| SPTB2 | 125 | 2 | 75 | 23 | 36 |
| VASP | 17 | 3 | 10 | 4 | 35 |
| ANLN | 61 | 0 | 25 | 0 | 35 |
| RLA0 | 11 | 4 | 6 | 1 | 25 |
| MYH14 | 52 | 0 | 48 | 0 | 22 |
| ENAH | 10 | 0 | 9 | 1 | 14 |
| SVIL | 29 | 6 | 18 | 0 | 12 |
| EF1G | 8 | 1 | 2 | 0 | 10 |
| NUCL | 12 | 3 | 2 | 0 | 9 |
| IF4A1 | 5 | 2 | 3 | 0 | 9 |
| SERA | 3 | 1 | 6 | 0 | 8 |
| DNJA1 | 4 | 0 | 2 | 1 | 7 |
| XPO2 | 8 | 0 | 4 | 0 | 6 |
| DOCK7 | 17 | 1 | 8 | 0 | 5 |
| SYEP | 11 | 1 | 6 | 0 | 5 |
| MY18A | 9 | 0 | 10 | 0 | 4 |
| PRKDC | 4 | 0 | 3 | 0 | 1 |
| Enriched in metaphase | M PAI | ||||
| PLEC | 249 | 1,405 | 0 | 638 | 197 |
| COF1 | 0 | 25 | 5 | 12 | 99 |
| GTF2I | 15 | 83 | 0 | 68 | 70 |
| VIME | 15 | 39 | 8 | 27 | 60 |
| ARPC2 | 1 | 14 | 2 | 18 | 47 |
| SRC8 | 5 | 21 | 6 | 34 | 45 |
| ARPC5 | 5 | 10 | 0 | 3 | 39 |
| WDR1 | 11 | 26 | 2 | 22 | 36 |
| ARC1B | 2 | 13 | 0 | 11 | 29 |
| HORN | 9 | 115 | 6 | 23 | 24 |
| RL13 | 1 | 5 | 1 | 3 | 17 |
| DDX5 | 7 | 18 | 1 | 4 | 16 |
| TRI27 | 0 | 3 | 0 | 9 | 14 |
| EPIPL | 0 | 118 | 0 | 39 | 14 |
| EF2 | 0 | 22 | 0 | 5 | 14 |
| RSSA | 2 | 6 | 0 | 3 | 14 |
| CS021 | 4 | 10 | 0 | 4 | 9 |
| TPX2 | 0 | 10 | 0 | 6 | 9 |
| C4BPA | 0 | 7 | 0 | 4 | 8 |
| HNRPU | 2 | 6 | 0 | 3 | 5 |
| AFAP1 | 1 | 4 | 0 | 4 | 5 |
| MICA3 | 1 | 3 | 0 | 5 | 4 |
Normalized total spectral count (normalization as in Figure 2C) for proteins enriched in the interphase (top part) and metaphase (bottom part) FAIs for the two mass spectrometry repeats (MS1 and MS2). The last column displays the mean protein abundance index (PAI), which is calculated as the ratio of the spectral count of the protein of interest, averaged between the two mass spectrometry repeats, and of the molecular weight of the protein. The PAI corrects for differences in the number of observable peptides due to protein size and is thus an adjusted readout of protein abundance.