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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Cancer Res. 2019 May 7;79(14):3662–3675. doi: 10.1158/0008-5472.CAN-18-3464

Figure 4. Dysbiosis enhances both local and systemic fibrosis within normal tumor-adjacent mammary tissue in advanced tumor-bearing animals.

Figure 4

C57BL/6 mice were treated as described in Figure 2A. A. Normal-adjacent mammary glands were harvested from BRPKp110 tumor- and non-tumor bearing mice, with or without established dysbiosis, at early (early mammary tissue; day 12) and advanced (advanced mammary tissue; day 27) timepoints after tumor initiation. BRPKp110 mammary tumors (B) and lungs (C) were harvested from advanced tumor-bearing animals with or without established dysbiosis. All tissues were formalin-fixed and paraffin-embedded, and sections were stained with PicroSirius Red. Quantification of staining intensity was calculated using Image J software.