Lipid metabolism gene expression in liver of juvenile black seabream (Acanthopagrus schlegelii) fed the experimental diets (▒, control; , high-fat diet (HFD); ≡, HFD + choline (3 g/kg); ‖‖, HFD + choline (6 g/kg); ///, HFD + choline (12 g/kg)) for 8 weeks. The control was used as the reference group, and the mRNA expression levels of target genes were normalised relative to the expression of β-actin. Values are means (n 3), with standard errors represented by vertical bars. a,b,cMean values for each gene with unlike letters were significantly different (P < 0·05). cpt1a, Carnitine palmitoyltransferase 1a; hsl, hormone-sensitive lipase; srebp-1, sterol regulatory element-binding protein-1; fas, fatty acid synthase; accα, acetyl-CoA carboxylase α.