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. 2019 Nov 22;8:e38. doi: 10.1017/jns.2019.34

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© The Author(s) 2019

This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 8. — Effects of lipopolysaccharide injection for 24 h on inflammation gene expression in liver of juvenile black seabream (Acanthopagrus schlegelii) after feeding the 8-week experimental diets (▒, control; , high-fat diet (HFD); ≡, HFD + choline (3 g/kg); ‖‖, HFD + choline (6 g/kg); ///, HFD + choline (12 g/kg)). The control was used as the reference group, and the mRNA expression levels of target genes were normalised relative to the expression of β-actin. Values are means (n 3), with standard errors represented by vertical bars. ^a,b,cMean values for each gene with unlike letters were significantly different (P < 0·05). tgfβ-1, Transforming growth factor β-1.

Inline graphic — Effects of lipopolysaccharide injection for 24 h on inflammation gene expression in liver of juvenile black seabream (Acanthopagrus schlegelii) after feeding the 8-week experimental diets (▒, control; , high-fat diet (HFD); ≡, HFD + choline (3 g/kg); ‖‖, HFD + choline (6 g/kg); ///, HFD + choline (12 g/kg)). The control was used as the reference group, and the mRNA expression levels of target genes were normalised relative to the expression of β-actin. Values are means (n 3), with standard errors represented by vertical bars. ^a,b,cMean values for each gene with unlike letters were significantly different (P < 0·05). tgfβ-1, Transforming growth factor β-1.