. 2019 Nov 22;8:e38. doi: 10.1017/jns.2019.34

Table 2.

Primers for real-time quantitative PCR for inflammation related genes and β-actin of black seabream (Acanthopagrus schlegelii)

Gene	Nucleotide sequence (5′–3′)	Size (bp)	GenBank reference or publication	Functions
il-1β	Forward: CATCTGGAGGCGGTGAA	231	JQ973887	Pro-inflammation cytokine
il-1β	Reverse: CGGTTTTGGTGGGAGGA			Pro-inflammation cytokine
tnfα	Forward: GTCCTGCTGTTTGCTTGG	154	AY335443	Pro-inflammation cytokine
tnfα	Reverse: AATGGATGGCTGCCTTGG			Pro-inflammation cytokine
nf-κb	Forward: AGCCCAAGGCACTCTAGACA	154	MK922543	Nuclear transcription factor
	Reverse: GTTCTGGGCAGCTGTAGAGG			Nuclear transcription factor
tgfβ-1	Forward: GGGTTTCCAACTTCGGC	209	Xue et al.⁽³⁹⁾	Anti-inflammation cytokine
tgfβ-1	Reverse: TTGTGTCCGTGGAGCGT			Anti-inflammation cytokine
il-10	Forward: TGTCAAACGGTTCCTTGCAG	172	MK922542	Anti-inflammation cytokine
	Reverse: GGCATCCTGGGCTTCTATCT			Anti-inflammation cytokine
accα	Forward: AGTAGCCTGATTCGTTGGT	154	KX066238	Lipogenesis pathway
accα	Reverse: AGTAGCCTGATTCGTTGGT			Lipogenesis pathway
fas	Forward: AAGAGCAGGGAGTGTTCGC	213	KX066240	Lipogenesis pathway
fas	Reverse: TGACGTGGTATTCAGCCGA			Lipogenesis pathway
srebp-1	Forward: TGGGGGTAGGAGTGAGTAG	247	KX066235	Lipogenesis pathway
srebp-1	Reverse: GTGAAGGGTCAGTGTTGGA			Lipogenesis pathway
cptla	Forward: TGCTCCTACACACTATTCCCA	203	KX078572	Lipolysis pathway
cptla	Reverse: CATCTGCTGCTCTATCTCCCG
hsl	Forward: AGCAACTAAGCCCTCCCCATC	179	KX066236	Lipolysis pathway
hsl	Reverse: TCTTCACCCAGTCCGACACAC
pparα	Forward: ACGACGCTTTCCTCTTCCC	183	KX066234	Lipolysis pathway
pparα	Reverse: GCCTCCCCCTGGTTTATTC
β-actin	Forward: ACCCAGATCATGTTCGAGACC	212	Jiao et al.⁽³⁸⁾	Housekeeping gene
β-actin	Reverse: ATGAGGTAGTCTGTGAGGTCG

tgfβ-1, Transforming growth factor β-1; accα, acetyl-CoA carboxylase α; fas, fatty acid synthase; srebp-1, sterol regulatory element-binding protein-1; cpt1a, carnitine palmitoyltransferase 1a; hsl, hormone-sensitive lipase.