Figure 2. Accumulation of EB at pH 5 and pH 7 by E. coli K-12 AG100 in the presence and absence of glucose 0.4%, with and without 25 μg/ml of PMZ. The real-time accumulation curves demonstrated a higher intracellular EB concentration without glucose at pH 7 compared to pH 5. The intracellular concentration of EB was significantly higher in the presence of PMZ at pH 7, in addition the PMZ treated sample exhibited lower EB accumulation at pH 5. The calculation of statistical significance and p-value was based on the relative fluorescence index (RFI) of the given sample. The correlation is significant: p≤0.001 and p=0.005.
