Figure 3. Inhibitory effects of minocycline in combination with resveratrol or quercetin on the expression of Cox2 (A), Tnfα (B) and Nos2 (C) mRNA in Porphyromonas gingivalis (PG)-LPS-stimulated RAW264.7 cells. The cells were pretreated for 30 min in 0.4 mM minocycline, 10 μM resveratrol or 10 μM quercetin, and 0.4 mM tetracycline with or without resveratrol or quercetin, and then incubated for 3.5 h with or without 100 ng/ml PGLPS. Total RNAs were prepared, cDNAs were synthesized, and the expression levels of Cox2, Tnfα and Nos2 mRNA were determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. *Significantly different at p<0.01 vs. LPS group.