Figure 4. Plasma-activated medium (PAM)-induced reactive oxygen species (ROS) participate in the apoptosis of Aspc1 cells. A: The viability of Aspc1 cells treated with/without PAM (16.4 kV-120 s) for 24 h. PAM, and N-acetyl cysteine (NAC) added to PAM were left in a water bath at 37˚C for 30 min before being used to treat Aspc1 cells. B: Flow cytometric analysis of ROS generation in Aspc1 cells exposed to PAM (16.4 kV-120 s) with and without NAC for 6 h. C: Caspase-3 and-7 activities in Aspc1 cells treated with PAM (16.4 kV-120 s) with and without NAC for 6 h. D: Western blot analysis was performed to check the expression of signal transducer and activator of transcription 3 (STAT3), p-STAT3, AKT serine/threonine kinase 1 (AKT) and p-AKT, in Aspc1 cells treated with PAM (16.4 kV-120 s) with and without NAC for 6 h. The data are represented as the mean±SD of three independent experiments. Significantly different at *p<0.05; ***p<0.001 vs. the control (Con).