Figure 11. Secretion of interleukin-22 (IL-22) by Escherichia coli cells transformed with plasmid pRSET containing the IL-22-green fluorescent protein transgene (pRSET-IL-22-GFP) plasmid. A: Level of IL-22 detected in culture medium collected at 48 h. B: Level of IL-22 in cell lysates from E. coli-IL-22 and Lactobacillus reuteri-IL-22. C: Biological activity of IL-22 produced by Lactobacillus-IL-22 using the induction of IL-10 in Colo205 cells. Significantly different at **p<0.01 versus E. coli-GFP; #p<0.05 versus Colo205; and ^p<0.01 versus control Lactobacillus.
