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. 2020 Jan 20;10:3120. doi: 10.3389/fimmu.2019.03120

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Copyright © 2020 Gonçalves, Rezende, Oliveira, Ribeiro, Fattori, Silva, Prazeres, Queiroz-Junior, Santana, Costa, Beltrami, Costa, Birbrair, Verri, Lopes, Cunha, Teixeira, Amaral and Pinho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Non-reactive status of glial cells and leucocytes adhesion in the spinal cord after resolution of acute inflammation in AIA model. Immunized mice received either i.a. injection of mBSA (10 μg/10 μl of PBS) to AIA induction or PBS (10 μl) in control group. (A) Dorsal horn of the spinal cord (DHSC). (B) Iba1 and (C) GFAP relative evaluation of gene expression in the dorsal horn extracted at different time points (n = 8). Dorsal horn samples from the control group were extracted 8 days after i.a. injection. The results are represented as mean ± SEM; to statistical analyses was used ANOVA following by Dunnett post-hoc analyses. A laminectomy was performed 8 days after i.a. injection of mBSA or PBS to evaluate (E) adherent leucocytes (D, representative photo on right) in dorsal microvasculature of lumbar intumescence (D, schematic picture on left) by intravital microscopy (n = 4). The results are represented as mean ± SEM; (n = 8) p = specified value in the graph compared to control group (PBS) using two tailed unpaired T-test.