Figure 3. Human umbilical vein endothelial cells were transfected with miR-383 suppressor or negative control (NC) for 24 h before 48 h activation by treatment with high glucose (HG). A, Reactive oxygen species (ROS) inside the cells was assessed by the oxidation of H2DCFDA to DCF (magnification bars: 40 µm). B, Quantification of ROS. C, Activity of superoxide dismutase (SOD1) and D, catalase (CAT). E, Activity of SOD1 and F, CAT in aortas of db/db mice. Data are reported as mean±SE of 3 independent experiments. **P<0.01 vs control or WT; ^#P<0.05 vs NC+HG group or db/db (ANOVA and Tukey's post hoc test). WT: wild type; Con: control.